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PHARMACEUTICAL MICROBIOLOGY

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About Course

• Study of all categories of microorganisims especially for the production of alchol antibiotics, vaccines, vitamins enzymes etc..

Objectives: Upon completion of the subject student shall be able to;
1. Understand methods of identification, cultivation and preservation of various microorganisms
2. To understand the importance and implementation of sterlization in pharmaceutical processing and industry
3. Learn sterility testing of pharmaceutical products.
4. Carried out microbiological standardization of Pharmaceuticals.
5. Understand the cell culture technology and its applications in pharmaceutical industries.

Course Content

UNIT 1

  • Introduction, history of microbiology, its branches, scope and its importance.
    00:00
  • HISTORY OF MICROBIOLOGY
    00:00
  • Introduction to Prokaryotes and Eukaryotes
    00:00
  • Study of ultra-structure and morphological classification of bacteria
    00:00
  • Nutritional Requirements,Raw Materials used for culture media
    00:00
  • physical factors for growth,growth curve
    00:00
  • Isolation techniques and preservation techniques
    00:00
  • cultivation of anaerobes
    00:00
  • Quantitative measurement of bacterial growth
    00:00
  • microscopy
    00:00

Unit-2
Identification of bacteria using staining techniques (simple, Gram’s &Acid fast staining) and biochemical tests (IMViC). Study of principle, procedure, merits, demerits and applications of physical, chemical gaseous,radiation and mechanical method of sterilization. Evaluation of the efficiency of sterilization methods. 79 Equipments employed in large scale sterilization. Sterility indicators.

unit-3
Study of morphology, classification, reproduction/replication and cultivation of Fungi and Viruses. Classification and mode of action of disinfectants Factors influencing disinfection, antiseptics and their evaluation. For bacteriostatic and bactericidal actions Evaluation of bactericidal & Bacteriostatic. Sterility testing of products (solids, liquids, ophthalmic and other sterile products) according to IP, BP and USP.

unit 4
Designing of aseptic area, laminar flow equipments; study of different sources of contamination in an aseptic area and methods of prevention, clean area classification. Principles and methods of different microbiological assay. Methods for standardization of antibiotics, vitamins and amino acids. Assessment of a new antibiotic

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