BP810 ET. PHARMACOLOGICAL SCREENING METHODS

About Course

Scope:This subject is designed to impart the basic knowledge of preclinical studies in
experimental animals including design, conduct and interpretations of results.
Objectives
Upon completion of the course the student shall be able to,

 Appreciate the applications of various commonly used laboratory animals.

 Appreciate and demonstrate the various screening methods used in preclinical
research
 Appreciate and demonstrate the importance of biostatistics and researchmethodology
 Design and execute a research hypothesis independently

Recommended Books (latest edition):
1. Fundamentals of experimental Pharmacology-by M.N.Ghosh
2. Hand book of Experimental Pharmacology-S.K.Kulakarni
3. CPCSEA guidelines for laboratory animal facility. 4. Drug discovery and Evaluation by Vogel H.G. 5. Drug Screening Methods by Suresh Kumar Gupta and S. K. Gupta
6. Introduction to biostatistics and research methods by PSS Sundar Rao and J
Richard

Course Content

Unit –I Laboratory Animals:
Study of CPCSEA and OECD guidelines for maintenance, breeding and conduct of experiments on laboratory animals, Common lab animals: Description and applications of different species and strains of animals. Popular transgenic and mutant animals. Techniques for collection of blood and common routes of drug administration in laboratory animals, Techniques of blood collection and euthanasia.

Unit –II Preclinical screening models a. Introduction:
Dose selection, calculation and conversions, preparation of drug solution/suspensions, grouping of animals and importance of sham negative and positive control groups. Rationale for selection of animal species and sex for the study.

Study of screening animal models for
Diuretics, nootropics, anti-Parkinson’s,antiasthmatics,

Preclinical screening models
: for CNS activity- analgesic, antipyretic,anti-inflammatory, general anaesthetics, sedative and hypnotics, antipsychotic, antidepressant, antiepileptic, antiparkinsonism, alzheimer’s disease

Unit –III Preclinical screening models
: for ANS activity, sympathomimetics, sympatholytics, parasympathomimetics, parasympatholytics, skeletal muscle relaxants, drugs acting on eye, local anaethetics

Unit –IV Preclinical screening models
for CVS activity- antihypertensives, diuretics, antiarrhythmic, antidyslepidemic, anti aggregatory, coagulants, and anticoagulants Preclinical screening models for other important drugs like antiulcer, antidiabetic, anticancer and antiasthmatics

Research methodology and Bio-statistics
Selection of research topic, review of literature, research hypothesis and study design Pre-clinical data analysis and interpretation using Students ‘t’ test and One-way ANOVA. Graphical representation of data

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About Course

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Course Content

Unit –II Preclinical screening models a. Introduction: Dose selection, calculation and conversions, preparation of drug solution/suspensions, grouping of animals and importance of sham negative and positive control groups. Rationale for selection of animal species and sex for the study.

Study of screening animal models for Diuretics, nootropics, anti-Parkinson’s,antiasthmatics,

Preclinical screening models : for CNS activity- analgesic, antipyretic,anti-inflammatory, general anaesthetics, sedative and hypnotics, antipsychotic, antidepressant, antiepileptic, antiparkinsonism, alzheimer’s disease

Unit –III Preclinical screening models : for ANS activity, sympathomimetics, sympatholytics, parasympathomimetics, parasympatholytics, skeletal muscle relaxants, drugs acting on eye, local anaethetics

Unit –IV Preclinical screening models for CVS activity- antihypertensives, diuretics, antiarrhythmic, antidyslepidemic, anti aggregatory, coagulants, and anticoagulants Preclinical screening models for other important drugs like antiulcer, antidiabetic, anticancer and antiasthmatics