BP 811 ET. ADVANCED INSTRUMENTATION TECHNIQUES

About Course

Scope: This subject deals with the application of instrumental methods in qualitative and
quantitative analysis of drugs. This subject is designed to impart advanced knowledge on
the principles and instrumentation of spectroscopic and chromatographic hyphenated
techniques. This also emphasizes on theoretical and practical knowledge on modern
analytical instruments that are used for drug testing. Objectives:Upon completion of the course the student shall be able to
 understand the advanced instruments used and its applications in drug analysis  understand the chromatographic separation and analysis of drugs.  understand the calibration of various analytical instruments  know analysis of drugs using various analytical instruments

Recommended Books (Latest Editions)
1. Instrumental Methods of Chemical Analysis by B.K Sharma
2. Organic spectroscopy by Y.R Sharma
3. Text book of Pharmaceutical Analysis by Kenneth A. Connors
4. Vogel’s Text book of Quantitative Chemical Analysis by A.I. Vogel
5. Practical Pharmaceutical Chemistry by A.H. Beckett and J.B. Stenlake
6. Organic Chemistry by I. L. Finar
7. Organic spectroscopy by William Kemp
8. Quantitative Analysis of Drugs by D. C. Garrett
9. Quantitative Analysis of Drugs in Pharmaceutical Formulations by P. D. Sethi
10. Spectrophotometric identification of Organic Compounds by Silverstein

Course Content

UNIT-I Nuclear Magnetic Resonance spectroscopy
Principles of H-NMR and C-NMR, chemical shift, factors affecting chemical shift, coupling constant, Spin - spin coupling, relaxation, instrumentation and applications

Mass Spectrometry
Principles, Fragmentation, Ionization techniques – Electron impact, chemical ionization, MALDI, FAB, Analyzers-Time of flight and Quadrupole, instrumentation, applications

UNIT-II Thermal Methods of Analysi
Principles, instrumentation and applications of ThermogravimetricAnalysis (TGA), Differential Thermal Analysis (DTA), Differential Scanning Calorimetry (DSC)

X-Ray Diffraction Methods
Origin of X-rays, basic aspects of crystals, Xray Crystallography, rotating crystal technique, single crystal diffraction,powder diffraction, structural elucidation and applications.

UNIT-III Calibration and validation
as per ICH and USFDA guideline

Calibration of following Instruments
Electronic balance, UV-Visible spectrophotometer, IR spectrophotometer, Fluorimeter, Flame Photometer, HPLC and GC

UNIT-IV Radio immune assay
Importance, various components, Principle, different methods, Limitation and Applications of Radio immuno assay

Extraction techniques
:General principle and procedure involved in the solid phase extraction and liquid-liquid extraction

UNIT-V 07 Hours Hyphenated technique
LC-MS/MS, GC-MS/MS, HPTLC-MS.

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Course Content

UNIT-I Nuclear Magnetic Resonance spectroscopy Principles of H-NMR and C-NMR, chemical shift, factors affecting chemical shift, coupling constant, Spin - spin coupling, relaxation, instrumentation and applications

Mass Spectrometry Principles, Fragmentation, Ionization techniques – Electron impact, chemical ionization, MALDI, FAB, Analyzers-Time of flight and Quadrupole, instrumentation, applications

UNIT-II Thermal Methods of Analysi Principles, instrumentation and applications of ThermogravimetricAnalysis (TGA), Differential Thermal Analysis (DTA), Differential Scanning Calorimetry (DSC)

X-Ray Diffraction Methods Origin of X-rays, basic aspects of crystals, Xray Crystallography, rotating crystal technique, single crystal diffraction,powder diffraction, structural elucidation and applications.

UNIT-III Calibration and validation as per ICH and USFDA guideline

Calibration of following Instruments Electronic balance, UV-Visible spectrophotometer, IR spectrophotometer, Fluorimeter, Flame Photometer, HPLC and GC

UNIT-IV Radio immune assay Importance, various components, Principle, different methods, Limitation and Applications of Radio immuno assay

Extraction techniques :General principle and procedure involved in the solid phase extraction and liquid-liquid extraction

UNIT-V 07 Hours Hyphenated technique LC-MS/MS, GC-MS/MS, HPTLC-MS.