About Course
Scope: This subject deals with the application of instrumental methods in qualitative and
quantitative analysis of drugs. This subject is designed to impart a fundamental
knowledge on the principles and instrumentation of spectroscopic and chromatographic
technique. This also emphasizes on theoretical and practical knowledge on modern
analytical instruments that are used for drug testing.
Objectives: Upon completion of the course the student shall be able to
Understand the interaction of matter with electromagnetic radiations and its
applications in drug analysis
Understand the chromatographic separation and analysis of drugs.
Perform quantitative & qualitative analysis of drugs using various analytical
instruments
Course Content
UNIT 1- UV VISIBLE SPECTROSCOPY & FLUORIMETRY
-
Inroduction of spectrocopy, EMR
00:00 -
colorimeter, electronic transitions
00:00 -
Electronic transitions
00:00 -
Beer lamberts law & derivation
00:00 -
Beer lamberts law & deviation
00:00 -
Instrumentation of UV visible spectrophotometer
00:00 -
applications and spectrophotometric titrations
00:00 -
multi component analysis
00:00 -
fluorimetry theory and concept
00:00 -
Factors affecting fluorimetry & quenching
00:00 -
Instrumentation & applications
00:00
UNIT-II-IR-Spectroscopy, Flame photomety, Atomic absorption Spectroscopy and nephloturbidometry
UNIT III-COLUMN, TLC, PAPER CHROMATOGRAPHY, ELECTROPHORESIS
UNIT IV-HPLC&GC
UNIT V- ION EXCHANGE CHROMATOGRAPHY, GEL FILTERATION CHROMATOGRAPHY, AFFINITY CHROMATOGRAPHY
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